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SRX6389229: GSM3916497: TSS whole body NE579 37S rep 1; Cryptococcus neoformans var. neoformans; OTHER
1 ILLUMINA (Illumina HiSeq 2500) run: 13.1M spots, 901.3M bases, 452.4Mb downloads

Submitted by: NCBI (GEO)
Study: Transcriptome of Cryptococcus neoformans and Cryptococcus deneoformans
PRJNA552283 • SRP212774 • All experiments • All runs
show Abstracthide Abstract
The goal of this study was to position all transcripts extremities in two species of Cryptococcus using TSS-Seq and QuantSeq 3' mRNA-Seq when cells are grown under different conditions. We analysed also the level of expression of each genes in the same condition using the same cell sample. All these data have spiked in using a fixed quantity of S. cereviae cells added just before DNA and RNA extraction. Overall design: total RNA and DNA were extracted different Cryptococcus wild type and mutant strains and sequenced. TSS and PAS were positioned and gene expression was evaluated.
Sample: TSS whole body NE579 37S rep 1
SAMN12187156 • SRS5048719 • All experiments • All runs
Organism: Cryptococcus neoformans var. neoformans
Library:
Instrument: Illumina HiSeq 2500
Strategy: OTHER
Source: TRANSCRIPTOMIC
Selection: other
Layout: SINGLE
Construction protocol: Cells were grown on YPD at 30°C in exponential phase. The Cryptococcus cell preparation was spiked in with one tenth (OD/OD) of S. cerevisiae strain FY834 cells grown in YPD at 30°C in stationary phase. TSS-Seq libraries preparations were performed starting with 75 µg of total RNA. Single 50 bases single end reads were obtained using an an Illumina HiSeq2500 instrument according to the manufacturer's instructions (Illumina).
Experiment attributes:
GEO Accession: GSM3916497
Links:
Runs: 1 run, 13.1M spots, 901.3M bases, 452.4Mb
Run# of Spots# of BasesSizePublished
SRR962716013,061,941901.3M452.4Mb2020-01-21

ID:
8462297

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